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. 2015 Jun 3;5:10837. doi: 10.1038/srep10837

Figure 2. Suppression of cell death in PsCRN115-expressing plants.

Figure 2

(a) Suppression of elicitor-triggered cell death in PsCRN115-transgenic N. benthamiana. The leaves were infiltrated with Agrobacterium GV3101 cells containing a PVX vector carrying PsAvh241, Bax, Avr3a/R3a, PsojNIP or PsCRN63. The photographs were taken 5 d after infiltration. (b) Confirmation of protein expression of the cell-death elicitors using Western blot analysis. Mouse monoclonal antibodies against the HA-epitope tag were used to detect expression of the cell-death elicitors fused to HA tags. (c) Heat-induced programmed cell death in transgenic N. benthamiana. Leaves of GFP- and PsCRN115-transgenic plants were detached and floated on water at room temperature or 50 °C for 40 min in the dark. The photographs were taken at 0, 12 and 24 h after treatment. The treated leaves were stained with trypan blue to visualise cell death. The results were reproducible in each transgenic line in at least three replicates and #13 was shown as an example.