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. 2015 Jun 3;5:10878. doi: 10.1038/srep10878

Figure 4. No significant association of the PLZF signal transduction pathway in GH expression.

Figure 4

a–c GH3 cells were transfected with scramble control si-RNA (control), PRR si-RNA (si-PRR), or PLZF si-RNA (si-PLZF) for 48 h. PRR, PLZF, Gh1 and Actb mRNA levels were quantified using real-time RT-PCR. d GH3 cells were transfected with either scramble control siRNA (control) or PLZF si-RNA (si-PLZF) for 48 h, and cell lysates were subjected to immunoblotting using anti-PLZF, anti-PRR, anti-GH, or anti-actin antibodies. e GH3 cells were transiently transfected with either control empty vector (pcDNA3) or PLZF-expressing vector (PLZF-pcDNA3) for 48 h, and cell lysates were subjected to immunoblotting using anti-PLZF, anti-PRR, anti-GH, or anti-actin antibodies. Representative blots are shown. Full-length blots were shown in supplementary figure 3.