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. 2014 Dec 18;5(12):e1573. doi: 10.1038/cddis.2014.526

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0

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Effect of advanced age on (a) β-galactosidase, (b and c) lipofuscin autofluorescence, (d and f, green color) lysosome vesicles, (e and f, red color) mitochondrial pool labeling in intact mouse cardiomyocytes, (g) citrate synthase (CS) activity in isolated heart mitochondria and (h) mitochondrial yield determined as mitochondrial protein with respect to total cardiac protein in subsarcolemmal (SSM) and interfibrillar (IFM) mitochondria isolated from mouse hearts. Mean±S.E.M. from n=14–25 replicates (5–10 hearts)