Figure 2.

RSV suppressed t-BHP-induced collapse of mitochondrial membrane potential in HUVECs. Sirt3 was knocked down by Sirt3 siRNA transfection as described in the Materials and Methods section. At 24-h post-transfection, cells were pretreated with RSV of 10 μM for 2 h, washed, and then treated with or without t-BHP of 80 μM for an additional 4 h. (a) Determination of Δψm was carried out using CLSM. Red fluorescence was emitted by JC-1 aggregates in healthy mitochondria with polarized inner mitochondrial membranes, whereas green fluorescence was emitted by cytosolic JC-1 monomers, indicating Δψm dissipation. Merged images indicate co-localization of JC-1 aggregates and monomers. (b) Δψm in each group was calculated as the ratio of red to green fluorescence. All results are presented as mean±S.E.M. of at least three independent experiments. ^bP<0.01 versus the control group; ^dP<0.01 versus the t-BHP-treated group; ^eP<0.05 versus RSV (10 μM) and t-BHP (80 μM) co-treated group with control siRNA transfection