FIGURE 2:

Mlc1 localizes to the bud neck independently of the septin ring and Myo1, but its localization to the ectopic cortical sites formed in the absence of the septin ring and actin filaments depends on Myo1. (A, B) Myo1 displays similar localization profiles to Mlc1 in a septin mutant regardless the presence of actin filaments. Cells of the strain YEF7155 (cdc12‑6 MYO1‑GFP CDC3‑mCherry) untreated (A) or treated (B) with LatA were analyzed by time-lapse microscopy (n = 4 for each condition). (C) Mlc1 localizes to the bud neck during cytokinesis in the absence of the septin ring and Myo1. Cells of the strain YEF7081 (cdc12‑6 myo1Δ GFP‑MLC1 CDC3‑mCherry) were analyzed by time-lapse microscopy (n = 6). (D) Localization of Mlc1 to the ectopic cortical sites in LatA‑treated septin mutant depends on Myo1. LatA‑treated cells of the same strain as in C were subjected to time-lapse analysis (n = 6). Arrow indicates GFP-Mlc1 at the bud neck. All cells were grown in SC‑Leu medium at 39°C. Scale bars, 2 μm.