FIGURE 5:

Myo1 plays a major role in targeting Mlc1 to the division site before cytokinesis. (A) Time‑lapse analysis of Mlc1 and Cdc3 at the bud neck during the cell cycle in WT (YEF7070; GFP‑MLC1 CDC3‑mCherry) and myo1Δ (YEF7132; myo1Δ GFP‑MLC1 CDC3‑mCherry) cells in the absence or presence of LatA. (B, C) Direct comparison of Mlc1 localization profiles at the bud neck of WT (YEF7070) and myo1Δ (YEF7132) cells in the absence (B) or presence (C) of LatA. Dashed lines indicate the beginning of the septin‑intensity drop during the cell cycle. All cells were grown in SC‑Leu medium at 25°C.