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. 2015 Apr 1;26(7):1211–1224. doi: 10.1091/mbc.E14-09-1363

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© 2015 Feng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Myo1, Myo2, and Myo4 are collectively required for the localization of Mlc1 to the bud neck before cytokinesis. (A, B) Direct comparisons of Mlc1 localization profiles at the bud neck of WT (YEF7435) vs. myo2IQ6Δ (YEF7094; myo2IQ6Δ GFP‑MLC1 CDC3‑mCherry) cells (A) and of WT (YEF7435) vs. myo4Δ (YEF7381; myo4Δ GFP‑MLC1 CDC3‑mCherry) cells (B). (C) Time‑lapse analysis of Mlc1 and Cdc3 at the bud neck during the cell cycle in WT (YEF7435) and myo1Δ myo2IQ6Δ myo4Δ (YEF7055; myo1Δ myo2IQ6Δ myo4Δ GFP‑MLC1 CDC3‑mCherry) cells in the absence or presence of LatA. (D, E) Direct comparisons of Mlc1 localization profiles at the bud neck of WT (YEF7435) and myo1Δ myo2IQ6Δ myo4Δ (YEF7055) cells in the absence (D) or presence (E) of LatA. All cells were grown in SC‑Leu medium at 25°C. Dashed lines indicate the beginning of the septin‑intensity drop during the cell cycle.