TABLE 1:
Yeast strains used in this study.
| Strain | Genotype | Source |
|---|---|---|
| CRY1 | a ade2-1oc can1-100 his3-11,15 leu2-3112 trp1-1 ura3-1 | Stevens and Davis (1998) |
| RSY21 | Like CRY1, except myo2IQ6Δ | Stevens and Davis (1998) |
| M-17 | a cdc12-6 leu2 ura3 | Caviston et al. (2003) |
| YEF473A | a his3 leu2 lys2 trp1 ura3 | Bi and Pringle (1996) |
| YEF1804 | Like YEF473A, except myo1Δ::KanMX6 | Bi et al. (1998) |
| YEF2692 | Like YEF473A, except bni1Δ::HIS3 | This study |
| YEF2697 | Like YEF473A, except bnr1Δ::HIS3 | This study |
| YEF3387 | Like CRY1, except myo2IQ6Δ myo1Δ::KanMX6 | This study |
| YEF6743 | Like YEF473A, except (P414‑ADH1‑MLC1) | This study |
| YEF6744 | Like YEF473A, except mlc1Δ::URA3-Kan (P414‑ADH1‑MLC1) | This study |
| YEF6757 | Like YEF473A, except GFP‑MLC1 (P414‑ADH1‑MLC1) | This study |
| YEF6800 | Like YEF473A, except GFP-MLC1 CDC3-mCherry::LEU2 | This study |
| YEF6866 | Like M‑17, except CDC12 | This study |
| YEF6870 | a cdc12-6 GFP‑MLC1::LEU2 | Derivative of M‑17 |
| YEF6884 | a cdc12-6 GFP‑MLC1::LEU2 CDC3-mCherry::URA3 | Derivative of YEF6870 |
| YEF6888 | a GFP-MLC1::LEU2 CDC3-mCherry::URA3 | Derivative of YEF6866 |
| YEF6951 | Like YEF473A, except CDC3-mCherry::URA3 | This study |
| YEF7016 | Like CRY1, except myo2IQ6Δ myo1Δ::NatMX myo4Δ::KanMX6 GFP‑MLC1::LEU2 | This study |
| YEF7055 | Like CRY1, except myo1Δ::NatMX myo2IQ6Δ myo4Δ::KanMX6 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7070 | Like YEF473A, except CDC3-mCherry::URA3 GFP-MLC1::LEU2 | This study |
| YEF7081 | Like YEF473A, except cdc12-6 myo1Δ::KanMX6 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7090 | Like CRY1, except myo2IQ6Δ CDC3-mCherry::TRP1 | This study |
| YEF7094 | Like CRY1, except myo2IQ6Δ CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7127 | Like YEF473A, except myo1Δ::KanMX6 CDC3-mCherry::TRP1 | This study |
| YEF7130 | Like CRY1, except myo2IQ6Δ myo4Δ::KanMX6 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7131 | Like CRY1, except myo2IQ6Δ myo1Δ::KanMX6 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7132 | Like YEF473A, except myo1Δ::KanMX6 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7155 | Like YEF473A, except cdc12-6 CDC3-mCherry::TRP1 MYO1-GFP-Kan | This study |
| YEF7184 | Like YEF473A, except bni1Δ::HIS3 CDC3-mCherry::TRP1 | This study |
| YEF7185 | Like YEF473A, except bnr1Δ::HIS3 CDC3-mCherry::TRP1 | This study |
| YEF7200 | Like YEF473A, except bnr1Δ::HIS3 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7201 | Like YEF473A, except bni1Δ::HIS3 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7208 | Like YEF473A, except CDC3-mCherry::URA3 [YCp111-GFP-MLC1-Nterm(1-81)] | This study |
| YEF7209 | Like YEF473A, except CDC3-mCherry::URA3 [YCp111-GFP-MLC1-Cterm(82-149)] | This study |
| YEF7245 | Like CRY1, except myo2IQ6Δ CDC3-mCherry::TRP1 [YCp111-GFP-MLC1-Cterm(82-149)] | This study |
| YEF7259 | Like YEF473A, except bni5Δ::KanMX6 CDC3-mCherry::URA3 GFP-MLC1::LEU2 | This study |
| YEF7381 | Like YEF473A, except myo4Δ::KanMX6 CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |
| YEF7435 | Like CRY1, except CDC3-mCherry::TRP1 GFP-MLC1::LEU2 | This study |