FIGURE 6:
Effect of Arp2/3 complex inhibition on the motility of PICK1-associated vesicles. HeLa cells expressing GFP-PICK1129-415 treated with the Arp2/3 complex inhibitor CK-666 (200 μM) or DMSO (control). First column, first frame of time-lapse sequence. Second column, maximum projection of frames 1–20 taken at 3-s intervals. Third column, overlay of the first two columns (see Supplemental Movie S8). Fourth column, zooms of the regions boxed in the third column. The plot on the right shows the quantification of vesicle speeds 30, 60, and 90 min after treatment with CK-666 or 60 min after treatment with DMSO. The differences between control and CK-666-treated cells are statistically significant (p < 0.02 or p < 0.0001, n = 42–85 tracks from six to nine cells).