TABLE 1:
Data collection and scattering-derived parameters.
| PICK1 | MBP-PICK1 | |
|---|---|---|
| Data collection | ||
| Beam line | CHESS F2 | CHESS G1 |
| Wavelength (Å) | 1.254 | 1.257 |
| q range (Å−1) | 0.0129–0.15 | 0.0066–0.2 |
| Exposure time (s) | 30 | 40 |
| Sample concentration (mg/ml) | 0.24–1.92 | 0.46–7.5 |
| Temperature (K) | 277 | 277 |
| Structural analysis | ||
| Sample concentration (mg/ml) | 0.96 | 3.75 |
| I(0) (cm−1) (from Guinier plot) | 6.94 ± 0.16 | 41.42 ± 0.68 |
| Rg (Å) (from Guinier plot) | 62.2 ± 1.39 | 83.83 ± 1.74 |
| I(0) (cm−1) [from P(r)] | 6.32 ± 0.03 | 41.38 ± 0.51 |
| Rg (Å) [from P(r)] | 59.5 ± 0.14 | 86.36 ± 1.02 |
| Dmax (Å) [from P(r)] | 171 | 266 |
| Molecular mass determination | ||
| Partial specific volume (cm3/g) | 0.746 | |
| Contrast (Δρ × 1010 cm−2) | 2.7 | |
| Molecular mass [from I(0)] (kDa) | 172.2 | |
| Theoretical mass of dimer (kDa) | 173.6 | |
| Software used | ||
| Primary data reduction | Raw | Raw |
| Data processing | Raw | Raw |
| Ab initio analysis | DAMMIF | |
| Averaging and validation | DAMAVER |