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. 2015 Apr 1;26(7):1395–1410. doi: 10.1091/mbc.E14-09-1413

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© 2015 Kirkland, Peterson, Still, Brueggeman, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — (A) Schematic of the wild-type silenced locus. (B) Schematic of the modified locus used in this study and the mating assay when the Gal4 DNA–binding domain alone is expressed in the cell, resulting in no repression of the a1 gene. (C) Schematic of the modified locus used in this study and the mating assay when Gal4-Sir1 is expressed in the cell, resulting in silencing of the a1 gene.