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. 2015 Apr 1;26(7):1395–1410. doi: 10.1091/mbc.E14-09-1413

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© 2015 Kirkland, Peterson, Still, Brueggeman, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Two-hybrid analysis of interactions between Sir proteins and repair proteins. (A) A wild-type strain was transformed with two different 2μ plasmids to coexpress Gal4-DBD fusions and Gal4-AD fusions. Growth on YMD plates lacking histidine was used to monitor interactions. (B) Two-hybrid analysis in strains lacking Sir proteins, using Gal4-Tel1 as bait.