Figure 4.

MiR-940 inhibits cell proliferation and promotes apoptosis by targeting Nestin. 5-8F cells were transfected with miR-940 precursor (miR-940) or control miRNA (NC), together with Nestin overexpression plasmid (pcDNA3.1-NES) or control plasmid (pcDNA3.1). (a) The cells were subjected to MTT analysis at different time points following transfection. Data are shown as mean±S.D. from three independent experiments. *P < 0.05, compared with NC+pcDNA3.1; ^##P< 0.01, compared with miR-940 + pcDNA3.1. (b) Twenty-four hours after transfection, cells were harvested and analyzed by PI/Annexin V staining and flow cytometry. (c) Caspase 3/7 activity in 5-8F cells was measured 48 h after co-transfection. *P<0.05, compared with respective control