Figure 4.

Proteomic analyses of differentially expressed proteins between HeLa and HeLa-R cells. (a) Representative 2-DE gel images of the HeLa and HeLa-R cells. A total of 42 spots (marked with arrow and number) were identified as differentially expressed and of these, 26 proteins were upregulated, whereas 16 proteins were downregulated in HeLa-R cells. (b) Forty-two distinct proteins were classified into 13 groups based on their biological functions: metabolism (35%), proteolysis (10%), apoptosis regulation (9%), electron transport/redox regulation (7%), molecular chaperone (5%), signal transduction (5%), transcription regulation (5%), calcium ion binding (5%) and so on. (c) The identified proteins were categorized into groups according to their subcellular locations. Among them, 57% of the proteins were located in the cytoplasm and others were located in the nucleus or membrane as indicated. (d) Western blot confirmation of six proteins (PKM2, LDHA, ALDOC, TPI1, MDH1, and UQCRC1). β-Actin was used as an equal loading control