Figure 3.

Omi represses GSK3β expression. (a) Western blotting analysis was performed, showing that GSK3β protein abundances were increased in the brains and spinal cord of mnd2 mice of 25 days compared with those in wild-type mice of the same asge. Densitometric analyses from three independent experiments were quantified by one-way ANOVA, *P<0.05. (b) Western blotting analysis was performed showing that HA-tag full-length Omi, but not HA alone or HA-tagged full-length S276C mutant Omi, decreased GSK3β protein abundance. Densitometric analyses from three independent experiments were quantified by one-way ANOVA, *P<0.05. (c) Western blotting analysis was performed showing that the knockdown of Omi increased GSK3β protein abundance in SH-SY5Y cells. Densitometric analyses from three independent experiments were quantified by one-way ANOVA, *P<0.05. (d) Real-time RT-qPCR assays were performed showing that the mRNA levels of GSK3β were not altered in the mnd2 mice of 25 days compared with those in wide-type mice of the same age. n=3–4 per group, Δ, no significance; one-way ANOVA