Figure 6.

LIF facilitates trophoblast outgrowth in extravillous explant cultures, migration and invasion of trophoblast cells through poFUT1. (A) Effect of LIF on outgrowth of trophoblast transfected with poFUT1 siRNA using extravillous explant culture. Whole mount immunofluorescent assay indicating the silencing of poFUT1 in the outgrowing. Bright-field images (a, f, and k) show the outgrowth of the explants. The boxed areas in (a, f, and k) are enlarged and shown in (b–d, gi, l–n), respectively ( × 40). The antibodies used for immunofluorescent staining indicated on the images. (B and C) Effect of LIF on invasion in poFUT1 siRNA or poFUT1 cDNA-transfected trophoblast cells. (D and E) Effect of LIF on MMP-2 activity by gelatin zymography and TIMP-1 and -2 expression by western blotting in poFUT1 siRNA or poFUT1 cDNA-transfected trophoblast cells