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. 2014 Aug 14;5(8):e1374. doi: 10.1038/cddis.2014.336

Figure 3.

Figure 3

The mAbED-C99 F(ab′)2 fragment and mAbED-C99 significantly block ADAM10-mediated α-cleavage of recombinant human wild-type APP protein. (a) Purified recombinant human wild-type full-length APP protein with Tags (C-terminal MYC/DDK; 260 ng) was incubated with IgG1 isotype control, mAbED-C99, or mAbED-C99 F(ab′)2 fragment (each at 1 μg) in the presence or absence of activated ADAM10 (Act. ADAM10; 1 unit) in a total volume of 50 μl justified with a buffer. Immediately following 1 h incubation at 37°C, the resulting incubation products were directly subjected to WB analysis using pAb751/770. (b) For WB quantitative analysis, band density ratio of α-CTF to full-length APP was analyzed and presented as mean±S.D. (**P<0.005). The WB result is representative of three independent experiments with similar results