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. 2014 Aug 14;5(8):e1378. doi: 10.1038/cddis.2014.339

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Effects of Snail knockdown on CdCl₂-induced cellular damage in HK-2 cells. Cells transfected with control siRNA, SNAI1 siRNA-1, or SNAI1 siRNA-2 were incubated with or without 20 μM CdCl₂ (Cd) for 12 h (a and b) or 30 h (c). Cell lysates were subjected to western blotting using antibodies against E-cadherin, Snail, and actin. Immunoblots shown are representative of at least three independent experiments (a). Phase-contrast micrographs were taken (b). The viability of cells was determined by trypan blue exclusion assay. Each value is the percentage of trypan blue-positive cells and reflects the mean±S.D. of three experiments with duplicate assays in each experiment. **P<0.01 versus CdCl₂-treated cells transfected with control siRNA (c)