Figure 3.

The protective effect of miR-302d on oxidant-induced cell death is not associated with the Keap1/Nrf2 pathway. (a) The expression of Nrf2 and Keap1 mRNA in miR-302d-transfected hADSCs was assessed by real-time PCR. (b) Western blot analysis was performed with the indicated antibodies. Protein was isolated from miR-302d-transfected hADSCs following CoCl₂ exposure for 20 h. The protein expressions were quantified and shown as the ratio of untreated miR-control (right panel). (c) Downregulation of Nrf2 expression by transfection of siR-Nrf2 was confirmed by real-time PCR. (d) Effect of Nrf2 siRNA on CoCl₂-induced cell death. siR-control or siR-Nrf2 was transfected into miR-302d-transfected hADSCs. Following treatment with CoCl₂, cell viability was assessed. **P<0.01 versus CoCl₂ treated miR-control. ^##P<0.01 versus siR-control. ^$P<0.05 versus untreated control. Data are shown as the mean±S.D. of three independent experiments