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. 2014 Aug 28;5(8):e1392. doi: 10.1038/cddis.2014.353

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Global methylation levels as determined by LUMA and pyrosequencing of the LINE-1 promoter after bisulfite conversion. Data are shown for both embryonic day 13.5 (E13.5) and post-natal day 4 (PN4). (a) LUMA-Liver, (b) LUMA-Brain, (c) LUMA-Kidney, (d) LINE-Liver, (e) LINE-Brain and (f) LINE-Kidney. White bars show global methylation within the control group, gray bars from the NK-depleted group and the black bars from the DC expanded group. Data represent mean±S.D.-derived from 10 to 15 embryos/pups per group. Significant differences are noted as *P<0.05 and **P<0.01 according to ANOVA followed by Tukey post-hoc test