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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: J Neurochem. 2014 Aug 21;131(5):625–633. doi: 10.1111/jnc.12844

Figure 4. Changes in autophagic flux following 2 or 24 hr treatment with 10 nM rotenone in the presence or absence of rapamycin or 3MA.

Figure 4

All flux assays were performed in the presence of 40 μM CQ. (A) Western blot analysis of autophagy protein LC3 following 10 nM rotenone treatment ± 1 μM rapamycin, or treatment ± 10 mM 3MA for 2 hr. (B) Western blot analysis of autophagy protein LC3 following 10 nM rotenone treatment ± 1 μM rapamycin or treatment ± 10 mM 3MA for 24 hr. Student t-test, *p<0.05 compared to CQ treatment without rotenone, 3MA or rapamycin, #p<0.05 compared to same treatment without rotenone.