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. Author manuscript; available in PMC: 2015 Jun 3.
Published in final edited form as: Mini Rev Med Chem. 2006 Aug;6(8):875–884. doi: 10.2174/138955706777934964

Fig. 2.

Fig. 2

Hippocampal slices from hibernating AGS survive significantly better than slices from euthermic AGS when cultured in the presence of AGS serum. Euthermic AGS include cold-adapted AGS that did not hibernate and AGS in interbout euthermy, A and B) On day 1 of hippocampal slice culture at 37°C, dentate gyms (DG) neurons from hibernating AGS survive the same as euthermic AGS neurons when cultured in media with (control) B-27 Supplement Minus AO (Invitrogen, Carlsbad, CA). Percent cell death is quantified via presidium iodide fluorescence intensity (n=9 slices/3 hibernating AGS and n=9 slices/3 euthermic AGS). Number of neurons in DG arc counted following NeuN immunocytochemistry to identify neurons (n=7 slices/3 hibernating AGS and n=8 slices/3 eutheimie AGS). C) On day 1 of culture at 37°C, DG neurons from hibernating AGS survive better than DG neurons from euthermic AGS when B-27 is replaced with scrum from hibernating or euthermic AGS (n=9 slices/3 hibernating AGS and n=9 slices/3 euthermic AGS), *p< 0.95. Data are expressed as mean ± SEM and were analyzed with two-way ANOVA. Significant (p< 0.05) main effects or interactions were followed by Holm-Sidak pairwise comparison post-hoc analysis using SigmaStat 3.0 (Systat Software, Inc., Point Richmond, CA).