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. 2015 Jun 3;10(6):e0126414. doi: 10.1371/journal.pone.0126414

Fig 1. Schematic representation of the experimental procedure used in this study.

Fig 1

Leaf explants of Coffea arabica (Ca) and C. canephora (Cc) were cultivated in the somatic embryogenesis (SE) induction media. In order to evaluate the factors involved in the inhibition of SE in Ca, the next steps were followed: 1. Ca and Cc explants were co-cultured together in the same medium. 2. The explants from Ca were discarded and the conditioned medium (CM) was used either fresh (CM-∆) or autoclaved (CM+∆) to culture the Cc explants. 3. The CM from seven days was fractionated with a 5 kDa cut-off membrane and the LmmCM obtained was added into the embryogenic cultures of Cc and Daucus carota (Dc). 4. The LmmCM was analyzed by GC-MS or UPLC-ESI-MS and the commercial identified phenolic compounds were added to Cc cultures. 5. The effect of LmmCM in DNA methylation was compared with that of the 5-azacytidine. Green circles: Cc leaf explants; yellow circles: Ca leaf explants; orange circles: Dc embryogenic cells.