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. Author manuscript; available in PMC: 2015 Jun 3.
Published in final edited form as: Nat Struct Mol Biol. 2009 Jan 11;16(2):159–167. doi: 10.1038/nsmb.1544

Figure 2.

Figure 2

Effect of the trinucleotide repeat tract orientation on stability. (a) Experimental design. The ARG2 locus on chromosome X, at which repeats are cloned, is depicted, along with the replication origin (ARS1010) and centromere location (CEN X). Strains used in this study contain the same repeat tract cloned either in the CTG orientation (the CTG sequence is the lagging-strand template) or in the CAG orientation (the CAG sequence is the lagging-strand template). (b) Orientation effect on growth rate on hydroxyurea (HU) plates compared to standard glucose plates (YPD). WT, wild type.