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. 2015 Jun 3;10(6):e0126919. doi: 10.1371/journal.pone.0126919

Fig 4. Effects of farnesol on embedded growth of an efg1/efg1 strain.

Fig 4

Photomicrographs taken in situ of HLC52 cells embedded in YPS agar containing 100 M farnesol after 2 days at 24°C. (A). Three focal planes illustrating three colony morphologies. Arrow, non-filamentous spindle-shaped colonies in the lower level of the matrix; Arrowhead, upper level colony with compact pseudohyphal filaments that do not produce yeast; Boxed region, a colony expressing arboreal-shaped branching hyphae that develop midlevel in the matrix. Bar = 2 mm. (B). Higher magnification of area such as boxed in (A). Masses of elongated opaque or opaque-like cells (brackets) produced through invasive pseudohyphal migration. Bar = 150 μm. (C). Higher magnification of a leading edge of the pseudohyphal expansion from (B). Bar = 10 μm. (D). An opaque-like cell aggregation illustrating the final maturation step of opaque-like cells from the pseudohyphae. Calcofluor white stain. Bar = 5 μm.