Table 3. PCR primer sequences used in this study.

Name	Sequence (5’ to 3’)	Notes
PN333	TCGGGAAGTTTCAGGGTACCCATGG	KpnI Flipper
PN334	TCAGGCTCTAACTCGAGTCTAGC	XhoI Flipper
PN335	TGGTGACAACTCGAGAATGGTTTGACG	XhoI Add Back Flipper
PN526	TTTTGAGCTCATTACACATACAATATGC	SacI Flipper
PN527	GCTTTGAGGACCGCGGATCTCATTATTC	SacII Flipper
PN185*	GATGCCAGGTTAGATACTTCGATTGTAGACG	HBR1 K66R mutation
PN224*	CAGGTACACCTTCTTGTGGGAAATCATCTC	HBR1 G19S mutation
PN266*	CAGGTACACCTGGGTGTTCTAAATCATCTC	HBR1 G21S mutation
PN442	GCTATCACAAGGTCCCGGTG	Detect; 81 nt 5’ of PN333
PN372	TGTTTTGTGGTGCCGTGCAAG	Detect; SAP2 promoter
PN357	TGTGCACCTATCCGACCAAGG	Detect; CaSAT1
PN615	TGCTGTGCGTGCTTTTATTC	Detect; 558 nt 3’ of PN526

*Paired with reverse complement