Fig 2. In vitro ALI differentiation replicates gene signature of healthy tissue ex vivo.

Venn diagram depicting the number of genes expressed in healthy esophageal tissue (FPKM > 2, n = 9,649) and the number of genes altered during air-liquid interface (ALI) differentiation (day 14 compared to day 8, n = 2,191) (P < 0.05, fold change > 2.0) (A). Clustered heatmap showing the log₂ FPKM values of the 1,610 genes in common to both data sets. Also shown are the 20 most dysregulated genes in cluster 1 (induced at day 14) (B). Expression (FPKM) correlation for the 1,610 genes in common between the healthy esophageal tissue and genes altered during ALI differentiation (C).