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. 2015 Jun 3;10(6):e0128527. doi: 10.1371/journal.pone.0128527

Fig 6. Seed coat characteristics of transgenic plants carrying the impermeable qHS1 allele.

Fig 6

A soybean cultivar with a permeable seed coat, Kariyutaka (KA), was transformed with the 6,273-bp genomic region containing the putative promoter and coding region of qHS1 from TA-HS. T2 plants of three transgenic T1 plants (KA-qHS1; T2-1 to T2-3; gray bars) were compared with the T2 line transformed with the pMDC123-GFP construct (KA-GFP; open bars), which possessed only the GFP cassette. A, Average percentage of hard seeds (n = 60–100); B, Average number of cracks per area (270 × 361 μm) in seed coats on the dorsal side of seeds (n = 40). Error bars indicate the maximum errors of estimates at 95%.