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A, A fragment of 443 bp harboring the SNP can be digested by PvuII in the impermeable TA-HS, but not in the permeable Tachinagaha. ND, not digested; D, digested. M, Ladder marker (DNA-035, TOYOBO, Tokyo, Japan). B, Variation of seed coat permeability in 194 cultivated accessions. C, Genotypic constitutions at the CAPS marker for 69 impermeable seeds produced by 28 cultivated accessions.
