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. 2015 Jun 3;10(6):e0128323. doi: 10.1371/journal.pone.0128323

Fig 3. Analysis of the mRNA, protein expression and proteolytic activities of the transformed and control Arabidopsis plants.

Fig 3

A. Analysis of the mRNA expression levels of T2 transformed lines and non-transformed control (Col) by RTq-PCR. Values expressed as the relative mRNA levels of the different transgenes were normalized to the Arabidopsis ubiquitin gene expression. B. Protein content detected by western-blot analysis of T2 transformed lines and non-transformed control (Col), using 10 μg of protein/lane and anti-GFP and anti-Rubisco antibodies. Large subunit of Rubisco (RbcL) was used as control loading. MW: molecular weights are indicated in kDa. C. Proteolytic activities of T2 transformed lines and non-transformed control (Col) expressed as nmoles/min/mg, using specific substrates of cathespin L- and B-like. Data are mean ± SE of triplicate measurements of each sample. Different letters indicate significant differences (P<0.05, Tukey's HSD). Transgenic plants were: SPM (lines 1.1, 1.2, 1.3), PM (lines 2.1, 2.2, 2.3) and P (lines 3.1, 3.3, 3.3).