Fig 3. Cx3cr1 promotes Aβ(1–42)-induced cytotoxicity, preferentially in hippocampal neurons.

(A) Aβ(1–42)-induced cell loss, measured by β-tubulin staining patterns of mature microglia-depleted cultured hippocampal neurons from C57Bl/6 (WT) or Cx3cr1 ^-/- (KO) mice. Scale bar, 10 μm. (B) Aβ(1–42)-induced cytotoxicity, quantified biochemically as % of maximal LDH release in microglial-depleted hippocampal and mixed cortical neurons. Maximum LDH release was defined using control neurons, or microglia respectively, treated with 2% Triton X-100. Results are summarized as the mean ± SEM of cumulative data from 7 separate experiments repeated in triplicate for each genotype. **** signifies P = 0.0001, two-way ANOVA. (C) Aβ(1–42)-induced cell loss, measured by tubulin staining patterns of pure microglia. Scale bar, 10 μm. (D) Aβ(1–42)-induced cytotoxicity, quantified biochemically as % of maximal LDH release in pure microglia cultures. Maximum LDH release was defined using control neurons, or microglia respectively, treated with 2% Triton X-100. Results are summarized as mean ± SEM of cumulative data from 7 separate experiments repeated in triplicate for each genotype. ****P = 0.0001, Mann-Whitney.