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. 2015 Jun 3;10(6):e0127730. doi: 10.1371/journal.pone.0127730

Fig 6. Aggregation state of recombinant met-Aβ(1–42) determines its ability to depress pre- or postsynaptic components of mEPSCs, and Cx3cr1 -/- hippocampal neurons are resistant to both effects.

Fig 6

(A) Non-HFIP recombinant met-Aβ(1–42) in wild-type hippocampal neurons. (B) HFIP recombinant met-Aβ(1–42) in wild-type hippocampal neurons. (C) Non-HFIP recombinant met-Aβ(1–42) in Cx3cr1 -/- hippocampal neurons. (D) HFIP recombinant met-Aβ(1–42) in Cx3cr1 -/- hippocampal neurons. For (A) through (D): the top three tracings are representative tracings of multiple mEPSCs recorded in the conditions indicated above each tracing. The single mEPSC is representative tracing of the average amplitude, rise- and decay-time of recordings from 6 independent experiments, each of which averaged recordings from 3 separate coverslips. Cumulative probabilities represent averages of all 6 experiments. For the bottom three graphs: control, solid black line; 200 nM Aβ(1–42), light blue line (A) and (D) and light green line (B) and (D); 2 μM Aβ(1–42), dark blue line (A) and (C) and dark green line (B) and (D). Recorded mEPSCs were abolished by 10 μM CNQX. For (A) through (D), the scale bars for multiple mEPSC tracings are: vertical, 20 pA and horizontal, 1 s; for multiple mEPSCs treated with CNQX: vertical, 50 pA and horizontal, 1 min; and for single mEPSC tracings: vertical, 20 pA and horizontal, 6 ms. See Table 2 for all parameters and P values. (E) Summary of mEPSC amplitudes and frequencies for synthetic (solid black circles) and recombinant (solid gray circles) Aβ(1–42)-treated wild-type neurons. (F) Summary of mEPSC amplitudes and frequencies for synthetic (solid black circles) and recombinant (solid gray circles) Aβ(1–42)-treated Cx3cr1 -/- neurons. Data were inspected for normality of distribution of frequency and amplitude values by the Kolmogorov-Smirnov test, analyzed for statistical significance by two-way ANOVA by genotype and treatment condition, with posthoc Bonferroni correction for multiple testing. Aβ concentrations and preparation protocols are indicated on the x-axes. Control, incubated with aCSF only. Data represent mean ± SEM of 6 independent experiments in each condition. P values for difference in parameters after treatment with synthetic (s) or recombinant (r) Aβ are shown. See Tables 2 and 3 for all parameters.

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