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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Arch Toxicol. 2014 Aug 1;89(8):1329–1336. doi: 10.1007/s00204-014-1321-8

Figure 2. Effect of PP2 treatment on CYP1A1 mRNA expression and CYP1A enzyme activity in NCTC-EV and NCTC-shAHR keratinocytes.

Figure 2

A. NCTC-EV and NCTC-shAHR cells were treated for 6h and 24h with 10µM PP2 or solvent. Transcription of CYP1A1 and β-actin was analyzed by quantitative real-time PCR. Results are shown as fold of solvent ctrl. *, significantly increased compared to solvent-treated samples. B. NCTC-EV cells were treated with PP2 (1µM, 5µM, 10µM), 10µM MNF, 10µM MNF plus 10µM PP2, 1µM BaP or solvent. After 24h EROD activities were measured. *, significantly increased compared to solvent-treated samples; #, significantly reduced compared to 10µM PP2-treated samples, §, significantly reduced compared to BaP-treated samples. C. NCTC-shAHR cells were treated with PP2 (1µM, 5µM, 10µM), 1µM BaP or solvent and EROD activities were measured after 24h. *, significantly reduced compared to solvent-treated NCTC-EV cells.