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. 2015 Jun 3;10(6):e0128700. doi: 10.1371/journal.pone.0128700

Fig 5. DNA translocation analysis by triplex displacement.

Fig 5

Reactions were examined by agarose gel electrophoresis and autoradiography of 32P-labelled-triplex-forming-oligonucleotide (Materials and Methods). Bound (triplex) and free triplex-forming-oligonucleotide (TFO) are indicated. Lane 1, free TFO; lane 2, triplex formed by the triplex-forming-oligonucleotide and covalently closed pLKS5 plasmid DNA; lane 3, triplex incubated with endonuclease reconstituted with wildtype HsdR; lane 4, triplex incubated with endonuclease reconstituted with mutant Lys220Ala HsdR, lane 5, triplex incubated with endonuclease reconstituted with mutant Lys220Glu HsdR; triplex incubated with endonuclease reconstituted with mutant Lys220Arg HsdR.