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. 2015 Jun 3;10(6):e0128679. doi: 10.1371/journal.pone.0128679

Fig 1. Vegetative explants collected from adult radiata pine trees for culture initiation.

Fig 1

A) Pre-flush shoot buds were collected from either adult somatic embryo-derived trees (PS-A and PS-B) or from four adult seed-derived trees. B) Apical sections of primordial shoots collected from the four seed-derived trees were cultured on LP1 medium containing 22 μM BA to promote axillary shoot development. Note the developing needle fascicles (7x). C) PGR-free LP1 medium was then used to promote elongation of axillary shoots, from which 2–3 mm slices were taken for SE induction (7x). D) Embryo development medium (EDM) containing four different PGR compositions (see Materials and Methods) was used for induction of tissues within sections from either primordial shoots collected from two genotypes of somatic embryo-derived trees (PS-A and PS-B), or axillary shoots developed in culture collected from four genotypes of seed-derived trees (Axl-C, Axl-D, Axl-E and Axl-F).