Table 2.
Primers used in this work.
| Primer name | Sequence (5′-3′) | Restriction site |
|---|---|---|
| KpnI-1752 | TAAGGTACCTCGCTGCGATCTTGCAGGGACT | KpnI |
| 1752-MfeI | TAACAATTGCGGCGGTCGCGGGCCACTAAA | MfeI |
| HindIII-1752 | TTAAAGCTTATGACTCACGAAATCGCCGTCATT | HindIII |
| HindIII-4049 | TTAAAGCTTATGAGCGCACTCGAAA | HindIII |
| 4049-MfeI | TATCAATTGCCGGTTACGCCACCCCGCG | MfeI |
| MfeI-amyH | AATCAATTGATCATATATGCCAACTATTTA | MfeI |
| amyH-PstI | AAACTGCAGTAGTGGAAAGCGAGCCAGC | PstI |
| leuA-F | ATGGCACGTTATCCGCCATCT | / |
| leuA-R | TCAGTCGTCGGCCGGCGT | / |
| leuC-F | ATGAGCGCGGAAACGCTG | / |
| leuC-R | TCATACCGAGGTCACCTCCT | / |
| leuD-F | ATGACGGACGAGGTCGAGATT | / |
| leuD-R | TACTCCGCTTCGGGAATCGC | / |
| leuB-F | GTGAGTATGACTCACGAAAT | / |
| leuB-R | TTACAGTCGGTCGATGAT | / |
| dapA-F | GCGACCCAAGAGACCACA | / |
| dapA-R | TCATCGCTCATCCTCCG | / |
| dapF-F | ATGAATATCCCATTTCAGAAGT | / |
| dapF-R | TCACGGCTCGGCCGGGGATT | / |
| lysA-F | ATGAACGAAACCCAAACGTT | / |
| lysA-R | TATCGGTCGTATTCGTTGTC | / |
| dapD-F | ATGAGCGCACTCGAAACCGA | / |
| dapD-R | TTACTCCCGCAGCGCGTCCT | / |
| dapB-F | ATGACGACGCGAATCGGCGTCAC | / |
| dapB-R | TCATTCGCTGATCACGTCTGCGAA | / |
| argF-F | ATGACGACCGATTCCGATCC | / |
| argF-R | TCACTCGAGCACCCAACTCA | / |
| argE-F | ATGAGTGCAACCATGGACGACA | / |
| argE-R | TCATTCGTCGTCCTCGCGAA | / |
| argD-F | ATGAGCGACCTCGATTTCGT | / |
| argD-R | TCATGCGTTGGAGTCGGT | / |
| argB-F | GGGCACCCTAACATGACGAC | / |
| argB-R | TCATTGGGCCACCTCC | / |
| argC-F | ATGGCGGTCGGGACCGAG | / |
| argC-R | TTAGGGTGCCCCCACGGG | / |
| argXW-F | ATGACCGAATGCGTCGAGTG | / |
| argXW-R | TCAGGCGGTCACCTC | / |
| argH-F | ATGACCGAGGAGAGCGCTCAC | / |
| argH-R | TCAGACATAGCCGTTCACCT | / |
| argG-F | GATATGACCCGCGTGGCACT | / |
| argG-R | TTACTGGTCGTCTCCCGTCCC | / |