Figure 3.

(a) Cell viability of SCL-1 tumor cells. Cells (SCL-1) were incubated with Fe₃O₄ nanoparticles. The percentage of living cells after 72 h was measured. n = 3. Ct: control (untreated). (b) Involvement of Fe₃O₄ nanoparticles in tumor invasion. Conditioned media of HDF (CM^HDF), myofibroblasts (CM^MF), and fibroblasts incubated with 50 μM or 350 μM Fe₃O₄ nanoparticles and rTGFβ1 (CM^{HDF,TGF,Fe₃O₄}) were used to check the invasive capacity of tumor cells. The data represent the mean ± s.e.m. n = 3. (c) Direct effect of Fe₃O₄ nanoparticles on tumor cells. SCL-1 tumor cells were untreated or incubated with 350 μm Fe₃O₄ nanoparticles for 24 h. The invasion assay was performed with conditioned media of HDF (CM^HDF) and myofibroblasts (CM^MF). The data represent the mean ± s.e.m. n = 3. ^* p < 0.05 versus CM^MF (Student's t-test). CM: conditioned medium.