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. 2015 May 21;2015:356893. doi: 10.1155/2015/356893

Figure 2.

Figure 2

Upregulation of VEGF mRNA and protein in OA cartilage samples. (a) qRT-PCR analysis was performed to examine MEG3 RNA levels in cartilage tissues from 20 OA patients and 10 controls. The results shown are from three independent qRT-PCR runs and are reported as the mean ± SD. P < 0.01. (b) The cartilage tissues from 20 OA patients and 10 controls were homogenized in buffer, and concentrations of immunoreactive VEGF were determined in homogenates by ELISA that detects all VEGF splice variants. The results shown are from three repetitions and are reported as the mean ± SD. P < 0.01.