Figure 2.

TSH increased mean mRNA expression of human normal thyroid follicular cells genes in primary culture. The sodium-iodide symporter NIS gene was evaluated after 4 h, and the thyroglobulin TG, thyroperoxidase TPO, and KI-67 genes were evaluated after 48 h. Data were obtained by RT-qPCR and normalized with β-actin gene expression and are shown as the mean ratio of TSH/no TSH ± standard deviation, considering no TSH = 1. The experiments were performed in duplicate and repeated in three independent cultures for NIS and four independent cultures for TG, TPO, and KI-67.