Table 6.
Copper deficiency (Cu−), copper excess (Cu+), cadmium excess (Cd+), and sulfur deficiency (S−) stresses affected Arabidopsis miRNAs revealed by high-throughput small RNA NGS.
| Cu− | Cu+ | Cd+ | |||
|---|---|---|---|---|---|
| MiRNA | FC | MiRNA | FC | MiRNA | FC |
| miR159a | 1.84 | miR169d,e,f-5p,g-5p | 0.79 | miR156f-3p | 2.84 |
| miR159b-3p | 2.44 | miR169a-3p | 0.29 | miR157a-3p,b-3p | 2.22 |
| miR162a,b-5p | 1.27 | miR319a,b | 3.27 | miR159b-3p | 2.11 |
| miR165a-3p,b | 1.56 | miR394a,b-5p | 3.59 | miR167c-3p | 1.58 |
| miR166a-3p,b-3p,c,d,e-3p,f,g | 1.51 | miR400 | 0.78 | miR319a,b | 3.14 |
| miR167c-3p | 1.86 | miR771 | 0.48 | miR396b-3p | 1.90 |
| miR168b-3p | 2.29 | miR829-5p | 0.25 | miR400 | 0.47 |
| miR169g-5p,f-5p,d,g | 0.70 | miR837-3p | 0.85 | miR847 | 2.52 |
| miR171a-5p | 3.40 | miR841a-3p | 1.56 | miR851-3p | 2.19 |
| miR319a,b | 2.36 | miR843 | 1.93 | miR3440b-5p | 5.29 |
| miR319b.2 | 1.40 | miR847 | 2.25 | miR5640 |  |
| miR396a-3p | 1.99 | miR5632-3p | 1.90 | miR5641 | 0.49 |
| miR400 | 0.54 | miR5664 | 1.58 | miR5996 | 1.21 |
| miR472-3p | 1.98 | miR8175 | 0.49 | miR8175 | 0.43 |
| miR781a,b | 0.34 | S− | |||
| miR830-5p | 0.16 | MiRNA | FC | MiRNA | FC |
| miR843 | 1.60 | miR173-5p | 0.77 | miR2111a-3p | 2.04 |
| miR847 | 3.14 | miR395a,d,e | 2.13 | miR5632-3p | |
| miR5012 | 0.21 | miR319b.2 | 1.22 | miR5638b | 0.44 |
| miR5642a,b | 1.63 | miR403-5p | 2.09 | miR8172 | 0.45 |
| miR8171 | 1.42 | miR771 | 0.28 | ||
| miR8175 | 0.29 | miR2111a-5p,b-5p | 1.27 | ||
NGS results were suggested by One-Way Anova test (p ≤ 0.05). MiRs: 5632-3p and 5640 levels could not be determined due to no read counts obtained under stress conditions. New Cu−, Cu+, Cd+, S− stresses responsive Arabidopsis miRNAs unknown until now are shown in bold. FC means fold change.