Table 3.

Mixed linear analysis of mutations in the BLN1 and BLN2 IQ domain and cysteines and their effect on binding with CaM^a.

Treatment	Mean YFP cell countsb	Estimatec	T-value	Adjusted P-valued
BLN1 and CaM	57.00	–	–	–
BLN1-C36G and CaM	2.33	54.67	17.28	4.37E-07
BLN1-C45G and CaM	0.33	56.67	17.91	3.30E-07
BLN1-Q30G and CaM	8.33	48.67	15.38	1.08E-06
BLN1-Q42G and CaM	2.67	54.33	17.17	4.59E-07
BLN2 and CaM	32.33	–	–	–
BLN2-C37G and CaM	1.00	31.33	6.13	3.85E-04
BLN2-C47G and CaM	1.33	31.00	6.06	4.19E-04
BLN2-Q30G and CaM	0.00	32.33	6.32	2.99E-04
BLN2-Q44G and CaM	2.33	30.00	5.87	5.43E-04

^aA mixed linear model analysis was done using PROC MIXED of the SAS Software. Contrasts were designed to test the differences between the control and the treatment with cell counts as the response.

^bRepresents mean of total cells exhibiting YFP from three independent biological replications. The appearance of YFP fluorescing cells were equivalent among mutant or wild-type bombarded constructs.

^cDifference between the least square means for the total YFP cells in control vs. the treatment.

^dP-values were adjusted for multiple testing the methods of Dunnett (1980) and Hsu (1992). Treatments with adjusted p ≤ 0.05 were considered significantly different from the BLN1, BLN2, and CaM wild-type control interactions.