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. 2015 May 6;12(5):4921–4941. doi: 10.3390/ijerph120504921

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Electrophoretic separation of the PCR products obtained from amplification of Amphora coffeaeformis chromosomal DNA with primer pairs: (1) specific for small-subunit rRNA; (2) UniE-F and UniE-R, specific for a portion of the gene coding for the diatom elongation factor eEF1-a and (3) UniS-F₁ and UniS-R₁, specific for a portion of the gene coding for the diatom silicic acid transporter (SIT). The DNA fragment shown in lane 3 represents the amplicon after purification by agarose gel extraction. Lane L contains the 100 bp DNA ladder (Fermentas).