Table 1.
Size distribution of the NM-300K dispersion in the various culture medium suspensions used in exposure studies characterised by dynamic light scattering (size distribution by intensity, volume and number).
| NM-300K Suspension (93.5 µg/mL) | Hydrodynamic Size by Intensity | Hydrodynamic Size by Volume | Hydrodynamic Size by Number | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Medium Type (Cells) | Temp (˚C) | Cl Ion (mM) | Cysteine */ Cystine Content (µM) |
Time (h) | PdI | Z-Av (d.nm ± sd) | Peak 1 (d.nm ± sd) (%) | Peak 2 (d.nm ± sd) (%) | Peak 1 (d.nm± sd) (%) | Peak 2 (d.nm± sd) (%) | Peak 1 (d.nm ± sd) (%) |
| MilliQ Water MEM(-) |
20 | − | − | T0 | 0.473 | 36 ± 7 | 6 ± 1 (14) | 54 ± 6 (86) | 4 ± 1 (98) | 25 ± 3 (2) | 4± 1 (100) |
| T24 | 0.484 | 36 ± 10 | 6 ± 1 (9) | 56 ± 3 (91) | 4 ± 2 (97) | 30 ± 4 (3) | 3 ±1 (100) | ||||
| 20 | 75.7 | 99 | T0 | 0.453 | 44 ± 12 | 6 ± 1 (10) | 62 ± 8 (90) | 5 ± 1 (95) | 30 ±6 (5) | 4 ± 1(100) | |
| T24 | 0.569 | 188 ± 72 | 467± 56 (54) | 42 ± 3 (46) | 29 ± 3 (90) | 570 ±30(10) | 24 ±3 (100) | ||||
| L-15 (RTL-W1 *) | 20 | 88.0 | 990 * | T0 | 0.418 | 34 ± 1 | 7 ± 1 (8) | 53 ± 3 (92) | 5.8 ± 1 (93) | 28 ± 3 (7) | 5 ± 1 (100) |
| T24 | 0.234 | 209 ±4 | 254 ± 27 (100) | 294 ± 36 (100) | 136 ± 13(100) | ||||||
| EMEM (pyr) (RTH-149) | 20 | 75.7 | 99 | T0 | 0.529 | 58 ± 2 | 9 ± 2 (9) | 70 ± 9 (91) | 7 ± 1 (95) | 24 ± 7 (5) | 5 ± 2(100) |
| T24 | 0.441 | 53 ± 1 | 9 ± 1 (11) | 83 ± 2 (89) | 8 ± 1 (96) | 23 ± 3 (4) | 6 ± 1(100) | ||||
| EMEM (NEAA) (RTG-2) | 20 | 75.7 | 99 | T0 | 0.455 | 53 ± 3 | 8 ± 1 (7) | 79 ± 7 (93) | 5 ± 1 (97) | 21 ± 7 (3) | 4 ± 1 (100) |
| T24 | 0.297 | 43 ± 1 | 7 ± 2 (5) | 64 ± 7 (95) | 6 ± 2 (92) | 26 ± 8 (8) | 5 ± 2 (100) | ||||
| M199 (primary hepatocytes) | 16 | 75.3 | 5 * /83 |
T0 | 0.430 | 35 ± 1 | 6 ± 2 (6) | 58 ± 3 (94) | 5 ± 1 (92) | 26 ± 6 (8) | 4 ± 1 (100) |
| T24 | 0.428 | 35 ± 1 | 6 ± 1 (8) | 58 ± 4 (92) | 5 ±1 (96) | 26 ± 2 (4) | 4 ± 1 (100) | ||||
| Mean size distribution | T0 | 0.458 | 45 ± 12 | 7.5 ± 1 (7) | 65 ± 12 (93) | 6 ± 1 (94) | 24 ± 3 (6) | 5 ± 1 (100) | |||
| * excluding RTL-W1 culture medium L-15 | T24 * | 0.389 | 44 ± 9 | 7.3 ± 2 (8) | 68 ± 13 (92) | 6 ± 2 (95) | 25 ± 2 (5) | 5 ± 1 (100) | |||