Table 2.

IC50 (µg /mL) values calculated from the different cytotoxicity assay systems in the rainbow trout cell lines and primary hepatocytes after exposure to AgNO₃ and NM-300K.

Assay system	AlamarBlue			CFDA-AM		NRU
	AgNO3	NM-300K		AgNO3	NM-300K	AgNO3	NM-300K
Cell line	IC50 (µg/mL)
RTL-W1	11	75.9		32.2	15.9	10.9	10.7
RTH-149	1.1	19.8		1.4	21.8	0.4	24.9
RTG-2	2.8	41.7		1.3	43.1	1.0	37.2
Primary hepatocytes	2.0	30.6		2.7	37.7	3.8	45.2