Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 4;13:38. doi: 10.1186/s12951-015-0097-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Feugang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Flow cytometry evaluations of sperm labeling and viability. Spermatozoa were labeled with 0 nM (Control), 0.1 nM (QD0.1−), and 1 nM (QD1− and QD1+) nanoparticles, followed by their incubation with FITC-PSA to the acrosome integrity or intactness. Proportions of 0%, 76 ± 4%, and 91 ± 2% spermatozoa were labeled with nanoparticles in the Control, QD0.1, QD1−, and QD1+ groups. The mean fluorescence intensities of nanoparticles-labeled spermatozoa (A) and proportions of spermatozoa with intact and damage acrosomes (B) were evaluated. Spermatozoa incubated with 0 and 10 µM calcium ionophore served as negative and positive controls, respectively. Columns (RFI, in A and QD+ intact acrosome, in B) with different letters differ significantly (ANOVA-1; p < 0.05). Data are mean ± SEM of four independent replicates.