Figure 5.
H1′–H6/H8 region of a 2D proton NOESY spectrum of the 39 nt hairpin showing sequential proton walks for residues C12–C23 and G24–A31. The C23H1′–H6 and C23H1′–G24H8 NOEs are missing from the walk because the C23H1′ and H2O resonances are close to each other. H1′–H6/H8 walk NOEs are labeled in blue. Adenine H2 signals are labeled with red dashed lines. H1′–adenine H2 NOEs are labeled in red with only the label of the residue for H1′. A G19H1′ (5.45 ppm)–A21H8 (7.96 ppm) NOE is labeled in orange. The spectrum was acquired at 20 °C and a mixing time of 350 ms with a WATERGATE pulse to suppress the water signal. In the secondary structure of the 39 nt hairpin, residues whose intraresidue H1′–H6/H8 NOEs were identified in the NOESY walks are labeled in blue. Spectrum and walks for residues G2−G10 and U33−U39 are in Figure S5 of the Supporting Information.