Skip to main content
. 2015 May 22;6:7118. doi: 10.1038/ncomms8118

Figure 2. AzCAs permit optical control of TRPV1 in HEK293T cells.

Figure 2

HEK293T cells expressing TRPV1–YFP were observed using whole-cell patch clamp electrophysiology after the application of an AzCA derivative (1 μM). Error bars were calculated as s.e.m. (a) Voltage ramps were applied under both λ=350 nm and λ=450 nm irradiation. Larger currents were observed under λ=350 nm irradiation (AzCA4). (b) AzCA2, AzCA3 and AzCA4 emerged as photoswitchable TRPV1 agonists. (c) When voltage clamped, photoswitching could be repeated over many cycles (AzCA3).