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Simultaneous monitoring of the FECO (a,d,g,j), normal optical microscope showing Newton's rings (c) and lipid domain localization (f,i,l) using FL-SFA before and during the contact time of FR4 (see Fig. 3b), and their schematics (b,e,h,k). (a–c) Bare mica–mica contact; (d–f) two bilayers before FR2; (g–i) hemifused bilayers right after high compression; and (j–l) hemifused bilayers at tc∼70 min.
