Figure 2.

Association between oestrogen receptor and TFF3 mRNAs in primary breast tumours. RNA was prepared from primary breast tumours. Ten microgram of breast tumour RNA were separated by gel electrophoresis and transferred to Hybond-N membranes, and TFF3 mRNA (600 nucleotides) was detected by hybridisation (A). The association between TFF3 mRNA and oestrogen receptor mRNA expression in each primary tumour is shown (B). The Pearson's coefficient and P value are indicated on the panel. RNA was reverse transcribed and amplified by PCR with the primer pairs for the oestrogen receptor (233 bp) and TFF3 (490 bp) mRNAs. The PCR products were separated by gel electrophoresis on a 3% agarose gel and stained with ethidium bromide (C).