FIG 5.

Growth of wild-type E. coli in the presence of transferrin is dependent on inoculum size. E. coli BW25113 and the ΔentF mutant were grown in low-iron medium with human apotransferrin and sodium bicarbonate. Open circles represent the ΔentF mutant started from low density and closed circles the ΔentF mutant started from high density. Open triangles represent the wild type started from low density and closed triangles the wild type started from high density. Open diamonds represent the wild type started from low density with Ent added, and closed diamonds represent the wild type started from high density with Ent added. Cultures were run in duplicate, and ranges are within the symbols.